Frontiers in Chemistry (Sep 2022)
Sesquiterpene lactones from Artemisia vulgaris L. as potential NO inhibitors in LPS-induced RAW264.7 macrophage cells
- Xiang-Yu Chen,
- Xiang-Yu Chen,
- Xiang-Yu Chen,
- Xiang-Yu Chen,
- Tao Liu,
- Tao Liu,
- Tao Liu,
- Tao Liu,
- Yu-Ze Hu,
- Tian-Tian Qiao,
- Tian-Tian Qiao,
- Tian-Tian Qiao,
- Tian-Tian Qiao,
- Xiu-Juan Wu,
- Xiu-Juan Wu,
- Xiu-Juan Wu,
- Xiu-Juan Wu,
- Ping-Hua Sun,
- Chui-Wen Qian,
- Chui-Wen Qian,
- Chui-Wen Qian,
- Chui-Wen Qian,
- Chui-Wen Qian,
- Zhe Ren,
- Zhe Ren,
- Zhe Ren,
- Zhe Ren,
- Zhe Ren,
- Jun-Xia Zheng,
- Yi-Fei Wang,
- Yi-Fei Wang,
- Yi-Fei Wang,
- Yi-Fei Wang,
- Yi-Fei Wang
Affiliations
- Xiang-Yu Chen
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Xiang-Yu Chen
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Xiang-Yu Chen
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Xiang-Yu Chen
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Tao Liu
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Tao Liu
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Tao Liu
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Tao Liu
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Yu-Ze Hu
- College of Pharmacy, Jinan University, Guangzhou, China
- Tian-Tian Qiao
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Tian-Tian Qiao
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Tian-Tian Qiao
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Tian-Tian Qiao
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Xiu-Juan Wu
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Xiu-Juan Wu
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Xiu-Juan Wu
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Xiu-Juan Wu
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Ping-Hua Sun
- College of Pharmacy, Jinan University, Guangzhou, China
- Chui-Wen Qian
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Chui-Wen Qian
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Chui-Wen Qian
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Chui-Wen Qian
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Chui-Wen Qian
- GuangZhou (Jinan) Biomedical Research and Development Center Co., Ltd., Guangzhou, China
- Zhe Ren
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Zhe Ren
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Zhe Ren
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Zhe Ren
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Zhe Ren
- GuangZhou (Jinan) Biomedical Research and Development Center Co., Ltd., Guangzhou, China
- Jun-Xia Zheng
- School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, China
- Yi-Fei Wang
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Yi-Fei Wang
- Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China
- Yi-Fei Wang
- Guangdong Provincial Biotechnology Drug & Engineering Technology Research Center, Guangzhou, China
- Yi-Fei Wang
- National Engineering Research Center of Genetic Medicine, Guangzhou, China
- Yi-Fei Wang
- GuangZhou (Jinan) Biomedical Research and Development Center Co., Ltd., Guangzhou, China
- DOI
- https://doi.org/10.3389/fchem.2022.948714
- Journal volume & issue
-
Vol. 10
Abstract
Twelve new guaianolide sesquiterpene lactones (1–12), along with ten known analogs (13–22) were isolated from an EtOH extract of the dried aerial parts of Artemisia vulgaris L. The new structures were elucidated via abundant spectroscopic data analyses (HRESIMS, IR, 1D, and 2D NMR), and the absolute configurations of these compounds were determined by X-ray crystallography and ECD calculations. The compounds (1−22) were identified as guaiane-type sesquiterpenes with characteristic α-methylene-γ-lactone and α,β-unsaturated carbonyl moieties. All compounds were tested for their inhibitory activity against NO production in lipopolysaccharide-stimulated RAW264.7 macrophages. The isolated sesquiterpenoids dose-dependently exhibited an NO production inhibitory activity by inhibiting the expression of inducible NO oxidase (iNOS) and cyclooxygenase-2 (COX-2) with IC50 values ranging from 1.0 to 3.6 μM. The inhibitory effect on the NO production of the compounds (1–4 and 6–22) is better than that of the positive control (dexamethasone). The different substitutions of compounds on C-8 influence anti-inflammatory effects, as evidenced by the in silico analysis of related binding interactions of new compounds (1–12) with iNOS.
Keywords
