Cytotoxic and genotoxic potential of Bulgarian Rosa alba L. essential oil – in vitro model study

Abstract

Rosa alba L., also known as the white oil-bearing rose, has been cultivated in relatively small areas of Europe – predominantly in the Rose Valley of Bulgaria. An increasing number of studies in vitro and in vivo, including clinical studies, have demonstrated the therapeutic efficacy of rose oils in recent years. However, little is known about the cytotoxic and genotoxic potential of the phytocomplex oil extract derived from Rosa alba L. The aim of the present work was to investigate the cytotoxicity and clastogenic effects of Rosa alba L. essential oil and its main constituent – geraniol, as well as citral A, associated with allergies and contact dermatitis. We used classical cytogenetic methods and comet assay. 1-Methyl-3-nitro-1-nitrosoguanidine was used as a standard mutagen. The data showed that R. alba L. essential oil (in concentrations up to 1000 μg/mL) did not exhibit a statistically significant cytotoxic effect. The essential oil did not significantly increase the level of mitotic disturbances (micronuclei and aneuploidy effects) and had no significant effect on the induction of chromatid aberrations, compared to the untreated control sample. Only geraniol and citral A (in the concentrations used) increased significantly the percentage of migrated DNA in the comet tail, compared to the whole oil extract. This study gives a reason to believe that R. alba L. oil has potential as a supplementary component in some therapeutic strategies. It would be harmless to normal cells and tissues, but with potential for additive or synergistic cytotoxicity against cancer cells.

Keywords

• Rose alba L. essential oil
• citral A
• geraniol
• cytotoxicity
• genotoxicity