Establishment of a human induced pluripotent stem cell line, JUCTCi012-A, from multiple symmetric lipomatosis (MSL) patient carrying a homozygous Arg707Trp (c.2119C > T) mutation in the MFN2 gene

Nidaa A. Ababneh; Dema Ali; Raghda Barham; Ban Al-Kurdi; Nour Sharar; Sabal Al Hadidi; Omar Qanno'; Abdee T. Ryalat; Bareqa Salah; Abdalla Awidi

Stem Cell Research (Oct 2020)

Establishment of a human induced pluripotent stem cell line, JUCTCi012-A, from multiple symmetric lipomatosis (MSL) patient carrying a homozygous Arg707Trp (c.2119C > T) mutation in the MFN2 gene

Nidaa A. Ababneh,
Dema Ali,
Raghda Barham,
Ban Al-Kurdi,
Nour Sharar,
Sabal Al Hadidi,
Omar Qanno',
Abdee T. Ryalat,
Bareqa Salah,
Abdalla Awidi

Affiliations

Nidaa A. Ababneh: Cell Therapy Center, the University of Jordan, Amman, Jordan; Corresponding author.
Dema Ali: Cell Therapy Center, the University of Jordan, Amman, Jordan
Raghda Barham: Cell Therapy Center, the University of Jordan, Amman, Jordan
Ban Al-Kurdi: Cell Therapy Center, the University of Jordan, Amman, Jordan
Nour Sharar: Cell Therapy Center, the University of Jordan, Amman, Jordan
Sabal Al Hadidi: Cell Therapy Center, the University of Jordan, Amman, Jordan
Omar Qanno': Eneral Surgery Department, Jordan University Hospital, The University of Jordan, Amman, Jordan
Abdee T. Ryalat: King Hussein Cancer Center, Amman, Jordan
Bareqa Salah: Eneral Surgery Department, Jordan University Hospital, The University of Jordan, Amman, Jordan
Abdalla Awidi: Cell Therapy Center, the University of Jordan, Amman, Jordan; Hemostasis and Thrombosis Laboratory, School of Medicine, the University of Jordan, Amman, Jordan; Department of Hematology and Oncology, Jordan University Hospital, Amman, Jordan

Journal volume & issue: Vol. 48
p. 101967

Abstract

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Induced pluripotent stem cells (iPSCs) were generated from skin fibroblasts collected from a 39-year-old multiple symmetric lipomatosis (MLS) female patient carrying a point mutation in MFN2 gene (c.2119C > T). The resulting iPSCs showed typical embryonic-like morphology, expressed pluripotency stem cell markers, retained the normal karyotype after reprogramming and showed the potential to differentiate into three germ layers. This iPSC line can be used for studying MSL disease mechanisms.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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