International Journal of Nanomedicine (Dec 2021)
TPGS2000-DOX Prodrug Micelles for Improving Breast Cancer Therapy
Abstract
Lan Tang,1 Wenhui Jiang,1 Lan Wu,1 Xiaolan Yu,1 Zheng He,1 Weiguang Shan,1 Lulu Fu,1 Zhenhai Zhang,2 Yunchun Zhao3 1College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, People’s Republic of China; 2Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, People’s Republic of China; 3Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of ChinaCorrespondence: Zhenhai ZhangHospital of Integrated Traditional Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210018, People’s Republic of ChinaTel +86 13758190403Email [email protected] ZhaoWomen’s Hospital, School of Medicine, Zhejiang University, 1, Xueshi Road, Hangzhou, 310016, People’s Republic of ChinaTel +86 15858178673Email [email protected]: Doxorubicin (DOX) is an anthracycline antibiotic that inhibits the growth of several solid and hematologic malignant tumors. Increasing the targeting ability of DOX and reducing the multi-drug resistance (MDR) of tumor cells to DOX are major aims for researchers.Purpose: In this study, to increase therapeutic efficiency, reduce the side effects and the MDR of tumor cells to DOX, D-alpha-tocopheryl polyethylene glycol 2000 succinate monoester (TPGS2000)-DOX prodrug micelles were developed by grafting DOX to TPGS2000 via an amide bond that release DOX in the slightly acidic conditions in tumor tissue.Materials and Methods: The TPGS2000-DOX micelles were constructed using polyethylene glycol 12-hydroxy stearate (Solutol HS15) as the carrier. The in vitro drug release profile and dilution stability of the nanomicelles were determined. The in vitro cytotoxicity and distribution of the nanomicelles in the tumor cells were also investigated. Moreover, we explored the therapeutic outcomes using the MCF-7/ADR tumor-bearing murine model.Results: The average particle size was approximately 30 nm with a narrow distribution, which was conducive for solid tumor accumulation. The results of in vivo imaging and in vitro cellular uptake assays demonstrated that the TPGS2000-DOX micelles increased the tumor-targeting ability and cellular uptake of DOX. The anticancer potential of TPGS2000-DOX micelles was higher than that of DOX, as revealed by in vitro cytotoxic assays with MCF-7/ADR cells and in vivo antitumor assays with MCF-7 tumor-bearing nude mice.Conclusion: TPGS2000-DOX prodrug micelles reverse the MDR of tumor cells, achieve passive targeting by forming nanomicelles, and subsequently enhance the efficacy and reduce the toxicity of DOX.Keywords: doxorubicin, D-alpha-tocopheryl polyethylene glycol 2000 succinate monoester, breast cancer, multi-drug resistance, polymer prodrug nanomedicine
