Ki67 Immunofluorescence on Bovine Cell Lines

Justine Marsolier; Souhila  Medjkane; Martine  Perichon; Jonathan Weitzman

doi:10.21769/BioProtoc.958

Bio-Protocol (Nov 2013)

Ki67 Immunofluorescence on Bovine Cell Lines

Justine Marsolier,
Souhila Medjkane,
Martine Perichon,
Jonathan Weitzman

Affiliations

Justine Marsolier: Epigenetics and Cell Fate, UMR 7216 CNRS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Souhila Medjkane: Epigenetics and Cell Fate, UMR 7216 CNRS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Martine Perichon: Epigenetics and Cell Fate, UMR 7216 CNRS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Jonathan Weitzman: Epigenetics and Cell Fate, UMR 7216 CNRS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France

DOI: https://doi.org/10.21769/BioProtoc.958
Journal volume & issue: Vol. 3, no. 21

Abstract

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This is a rapid protocol to test the effects of drugs treatment on bovine cell replication using Ki67 staining. Ki67 is associated with cell proliferation and is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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