Frontiers in Plant Science (Jan 2023)
Genome-wide identification and expression analysis of AUX/LAX family genes in Chinese hickory (Carya cathayensis Sarg.) Under various abiotic stresses and grafting
- Ying Yang,
- Ying Yang,
- Jiayan Wang,
- Jiayan Wang,
- Yan Xu,
- Yan Xu,
- Farhat Abbas,
- Farhat Abbas,
- Dongbin Xu,
- Dongbin Xu,
- Shenchen Tao,
- Shenchen Tao,
- Xiaoting Xie,
- Xiaoting Xie,
- Feng Song,
- Feng Song,
- Qiaoyu Huang,
- Qiaoyu Huang,
- Anket Sharma,
- Anket Sharma,
- Luqing Zheng,
- Luqing Zheng,
- Daoliang Yan,
- Daoliang Yan,
- Xiaofei Wang,
- Xiaofei Wang,
- Bingsong Zheng,
- Bingsong Zheng,
- Huwei Yuan,
- Huwei Yuan,
- Rongling Wu,
- Rongling Wu,
- Yi He,
- Yi He
Affiliations
- Ying Yang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Ying Yang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Jiayan Wang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Jiayan Wang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Yan Xu
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Yan Xu
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Farhat Abbas
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Farhat Abbas
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Dongbin Xu
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Dongbin Xu
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Shenchen Tao
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Shenchen Tao
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Xiaoting Xie
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Xiaoting Xie
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Feng Song
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Feng Song
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Qiaoyu Huang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Qiaoyu Huang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Anket Sharma
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Anket Sharma
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Luqing Zheng
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Luqing Zheng
- College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, China
- Daoliang Yan
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Daoliang Yan
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Xiaofei Wang
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Xiaofei Wang
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Bingsong Zheng
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Bingsong Zheng
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Huwei Yuan
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Huwei Yuan
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Rongling Wu
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Rongling Wu
- Departments of Public Health Sciences and Statistics, Center for Statistical Genetics, Pennsylvania State University, Hershey, PA, United States
- Yi He
- State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- Yi He
- Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Functions, Zhejiang Agriculture and Forestry (A&F) University, Hangzhou, China
- DOI
- https://doi.org/10.3389/fpls.2022.1060965
- Journal volume & issue
-
Vol. 13
Abstract
Auxin is essential for regulating plant growth and development as well as the response of plants to abiotic stresses. AUX/LAX proteins are auxin influx transporters belonging to the amino acid permease family of proton-driven transporters, and are involved in the transport of indole-3-acetic acid (IAA). However, how AUX/LAX genes respond to abiotic stresses in Chinese hickory is less studied. For the first time identification, structural characteristics as well as gene expression analysis of the AUX/LAX gene family in Chinese hickory were conducted by using techniques of gene cloning and real-time fluorescent quantitative PCR. Eight CcAUX/LAXs were identified in Chinese hickory, all of which had the conserved structural characteristics of AUX/LAXs. CcAUX/LAXs were most closely related to their homologous proteins in Populus trichocarpa , which was in consistence with their common taxonomic character of woody trees. CcAUX/LAXs exhibited different expression profiles in different tissues, indicating their varying roles during growth and development. A number of light-, hormone-, and abiotic stress responsive cis-acting regulatory elements were detected on the promoters of CcAUX/LAX genes. CcAUX/LAX genes responded differently to drought and salt stress treatments to varying degrees. Furthermore, CcAUX/LAX genes exhibited complex expression changes during Chinese hickory grafting. These findings not only provide a valuable resource for further functional validation of CcAUX/LAXs, but also contribute to a better understanding of their potential regulatory functions during grafting and abiotic stress treatments in Chinese hickory.
Keywords
