Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi
Klaus Ringsborg Westphal,
Katrine Amalie Hamborg Nielsen,
Rasmus Dam Wollenberg,
Mathias Bonde Møllehøj,
Simone Bachleitner,
Lena Studt,
Erik Lysøe,
Henriette Giese,
Reinhard Wimmer,
Jens Laurids Sørensen,
Teis Esben Sondergaard
Affiliations
Klaus Ringsborg Westphal
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Katrine Amalie Hamborg Nielsen
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Rasmus Dam Wollenberg
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Mathias Bonde Møllehøj
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Simone Bachleitner
Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Straße 24, Tulln an der Donau 3430, Austria
Lena Studt
Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Straße 24, Tulln an der Donau 3430, Austria
Erik Lysøe
Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, 1433 Ås, Norway
Henriette Giese
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Reinhard Wimmer
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Jens Laurids Sørensen
Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark
Teis Esben Sondergaard
Department of Chemistry and Bioscience, Aalborg University, 9220 Aalborg, Denmark
Fungal non-ribosomal peptide synthetase (NRPS) clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one NRPS. However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in Fusarium graminearum. Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase kmt6, which induced the production of fusaoctaxin A.
