Measuring Endogenous GA and IAA

Yunping Chen; Zhiwu Dan; shaoqing Li

doi:10.21769/BioProtoc.4332

Bio-Protocol (Feb 2022)

Measuring Endogenous GA and IAA

Yunping Chen,
Zhiwu Dan,
shaoqing Li

Affiliations

Yunping Chen: State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China
Zhiwu Dan: State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China
shaoqing Li: State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan, China

DOI: https://doi.org/10.21769/BioProtoc.4332
Journal volume & issue: Vol. 12, no. 4

Abstract

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Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system. This protocol describes a detailed procedure to extract and quantify indole-3-acetic acid (IAA) and gibberellin acid (GA) in rice (Oryza sativa) tissues using high-performance liquid chromatography (HPLC)-based method.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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