Development and Immunoprotection of Bacterium-like Particle Vaccine against Infectious Bronchitis in Chickens
Pengju Zhang,
Tiantian Yang,
Yixue Sun,
Haiying Qiao,
Nianzhi Hu,
Xintao Li,
Weixia Wang,
Lichun Zhang,
Yanlong Cong
Affiliations
Pengju Zhang
Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Tiantian Yang
Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China
Yixue Sun
Department of Policies and Regulations, Changchun University, Changchun 130022, China
Haiying Qiao
Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Nianzhi Hu
Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Xintao Li
Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Weixia Wang
Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Lichun Zhang
Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
Yanlong Cong
Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China
Infectious bronchitis (IB) is a major threat to the global poultry industry. Despite the availability of commercial vaccines, the IB epidemic has not been effectively controlled. The exploration of novel IBV vaccines may provide a new way to prevent and control IB. In this study, BLP-S1, a bacterium-like particle displaying the S1 subunit of infectious bronchitis virus (IBV), was constructed using the GEM-PA surface display system. The immunoprotective efficacy results showed that BLP-S1 can effectively induce specific IgG and sIgA immune responses, providing a protection rate of 90% against IBV infection in 14-day-old commercial chickens. These results suggest that BLP-S1 has potential for the development of novel vaccines with good immunogenicity and immunoprotection.
