See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity

Jeong Hyun Lee; Emily S. Park; Jane Ru Choi; Kerryn Matthews; Alice V. Lam; Xiaoyan Deng; Simon P. Duffy; Hongshen Ma

doi:10.1038/s42003-022-03703-3

Communications Biology (Jul 2022)

See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity

Jeong Hyun Lee,
Emily S. Park,
Jane Ru Choi,
Kerryn Matthews,
Alice V. Lam,
Xiaoyan Deng,
Simon P. Duffy,
Hongshen Ma

Affiliations

Jeong Hyun Lee: Department of Mechanical Engineering, University of British Columbia
Emily S. Park: Department of Mechanical Engineering, University of British Columbia
Jane Ru Choi: Department of Mechanical Engineering, University of British Columbia
Kerryn Matthews: Department of Mechanical Engineering, University of British Columbia
Alice V. Lam: Department of Mechanical Engineering, University of British Columbia
Xiaoyan Deng: Department of Mechanical Engineering, University of British Columbia
Simon P. Duffy: Department of Mechanical Engineering, University of British Columbia
Hongshen Ma: Department of Mechanical Engineering, University of British Columbia

DOI: https://doi.org/10.1038/s42003-022-03703-3
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 10

Abstract

Read online

A selective sequencing method, termed SeeN-Seq, is presented to link morphological phenotypes to single-cell RNAseq using UV laser patterning of hydrogel porosity.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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