Nature Communications (Mar 2022)
BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation
- Andrew Keniry,
- Natasha Jansz,
- Linden J. Gearing,
- Iromi Wanigasuriya,
- Joseph Chen,
- Christian M. Nefzger,
- Peter F. Hickey,
- Quentin Gouil,
- Joy Liu,
- Kelsey A. Breslin,
- Megan Iminitoff,
- Tamara Beck,
- Andres Tapia del Fierro,
- Lachlan Whitehead,
- Andrew Jarratt,
- Sarah A. Kinkel,
- Phillippa C. Taberlay,
- Tracy Willson,
- Miha Pakusch,
- Matthew E. Ritchie,
- Douglas J. Hilton,
- Jose M. Polo,
- Marnie E. Blewitt
Affiliations
- Andrew Keniry
- The Walter and Eliza Hall Institute of Medical Research
- Natasha Jansz
- The Walter and Eliza Hall Institute of Medical Research
- Linden J. Gearing
- The Walter and Eliza Hall Institute of Medical Research
- Iromi Wanigasuriya
- The Walter and Eliza Hall Institute of Medical Research
- Joseph Chen
- Department of Anatomy and Developmental Biology, Monash University
- Christian M. Nefzger
- Department of Anatomy and Developmental Biology, Monash University
- Peter F. Hickey
- The Walter and Eliza Hall Institute of Medical Research
- Quentin Gouil
- The Walter and Eliza Hall Institute of Medical Research
- Joy Liu
- The Walter and Eliza Hall Institute of Medical Research
- Kelsey A. Breslin
- The Walter and Eliza Hall Institute of Medical Research
- Megan Iminitoff
- The Walter and Eliza Hall Institute of Medical Research
- Tamara Beck
- The Walter and Eliza Hall Institute of Medical Research
- Andres Tapia del Fierro
- The Walter and Eliza Hall Institute of Medical Research
- Lachlan Whitehead
- The Walter and Eliza Hall Institute of Medical Research
- Andrew Jarratt
- The Walter and Eliza Hall Institute of Medical Research
- Sarah A. Kinkel
- The Walter and Eliza Hall Institute of Medical Research
- Phillippa C. Taberlay
- School of Medicine, College of Health and Medicine, University of Tasmania
- Tracy Willson
- The Walter and Eliza Hall Institute of Medical Research
- Miha Pakusch
- The Walter and Eliza Hall Institute of Medical Research
- Matthew E. Ritchie
- The Walter and Eliza Hall Institute of Medical Research
- Douglas J. Hilton
- The Walter and Eliza Hall Institute of Medical Research
- Jose M. Polo
- Department of Anatomy and Developmental Biology, Monash University
- Marnie E. Blewitt
- The Walter and Eliza Hall Institute of Medical Research
- DOI
- https://doi.org/10.1038/s41467-022-29333-1
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 15
Abstract
Female embryonic stem cells (ESCs) are the ideal model to study X chromosome inactivation (XCI) establishment; however, these cells are challenging to keep in culture. Here the authors create fluorescent ‘Xmas’ reporter mice as a renewable source of ESCs and show nucleosome remodelers Smarcc1 and Smarca4 create a nucleosome-free promoter region prior to the establishment of silencing.
