Journal of Inflammation Research (Jan 2022)

The Potential Role of Serum IGF-1 and Leptin as Biomarkers: Towards Screening for and Diagnosing Postmenopausal Osteoporosis

  • Zhang Y,
  • Huang X,
  • Sun K,
  • Li M,
  • Wang X,
  • Han T,
  • Shen H,
  • Qi B,
  • Xie Y,
  • Wei X

Journal volume & issue
Vol. Volume 15
pp. 533 – 543

Abstract

Read online

Yili Zhang,1,* Xinyi Huang,2,* Kai Sun,3,* Mengyuan Li,1,* Xu Wang,3 Tao Han,3 Hao Shen,4 Baoyu Qi,3 Yanming Xie,5 Xu Wei3 1School of Traditional Chinese Medicine & School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, People’s Republic of China; 2School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, People’s Republic of China; 3Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China; 4Changxindian Community Health Service Center, Beijing, People’s Republic of China; 5Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yanming Xie; Xu Wei, Tel +86 13911112416; +86 13488716557, Email [email protected]; [email protected]: To investigate the differences of several serum markers among population with different bone mass and to explore the utility of new potential biomarker for the diagnosing and screening for postmenopausal osteoporosis (PMOP).Materials and Methods: A total of 1055 postmenopausal women were screened and gathered data on BMD screening, biological samples, and questionnaire information. A liquid chip assay was used to measure serum IL-6, IGF-1, BMP-2, VEGF, leptin and FGF23. The predictive value of the indicator panels was assessed using the area under the receiver-operator characteristic curve (AUC). Statistical analyses were conducted by using SAS 9.4 and R software 4.1.1. Figures were created in GraphPad Prism 8.0.Results: When compared against the normal group, in addition to the vitamin D, the PMOP group showed a significant increase in median values for other indicators (P < 0.05), especially in P1NP and β-CTX. Among the six cytokines representing different osteoporosis mechanisms, currently, we found that only IGF-1 and leptin showed significant differences between the groups. Also, the liquid chip assay results showed that IGF-1 and leptin, as newer cytokines in osteoporosis, not only have significant differences between groups, but also have a strong correlation with each other (P < 0.05). Then, we reported the accuracy of different indicator combinations by using AUC and, moreover, we demonstrated that IGF-1 with leptin did significantly provide incremental value to the AUC of conventional indexes, it markedly improved diagnostic efficacy, displaying an IDI of 9.45% (P = 0.000).Conclusion: IFG-1 and leptin seem to be key biomarker associated with PMOP. The high prevalence of PMOP makes these cytokines might bear the potential of becoming a very useful screening test also for clinical follow-up of patients.Keywords: biomarkers, menopause, diagnose, cytokine

Keywords