T cells regulate lymph node-resident ILC populations in a tissue and subset-specific way
Priscillia Bresler,
Emmanuel Tejerina,
Jean Marie Jacob,
Agnès Legrand,
Véronique Quellec,
Sophie Ezine,
Lucie Peduto,
Marie Cherrier
Affiliations
Priscillia Bresler
Institut Necker Enfants Malades, Université Paris Descartes, INSERM U1151, CNRS UMR 8253, Faculté de Médecine Necker, 156 rue de Vaugirard, 75015 Paris, France
Emmanuel Tejerina
Institut Necker Enfants Malades, Université Paris Descartes, INSERM U1151, CNRS UMR 8253, Faculté de Médecine Necker, 156 rue de Vaugirard, 75015 Paris, France
Jean Marie Jacob
Stroma, Inflammation & Tissue Repair Unit, Institut Pasteur, Inserm U1224, Paris, France; Université Paris Diderot, Sorbonne Paris Cité, Paris, France
Agnès Legrand
Institut Necker Enfants Malades, Université Paris Descartes, INSERM U1151, CNRS UMR 8253, Faculté de Médecine Necker, 156 rue de Vaugirard, 75015 Paris, France
Véronique Quellec
Institut Necker Enfants Malades, Université Paris Descartes, INSERM U1151, CNRS UMR 8253, Faculté de Médecine Necker, 156 rue de Vaugirard, 75015 Paris, France
Sophie Ezine
Institut Necker Enfants Malades, Université Paris Descartes, INSERM U1151, CNRS UMR 8253, Faculté de Médecine Necker, 156 rue de Vaugirard, 75015 Paris, France
Lucie Peduto
Stroma, Inflammation & Tissue Repair Unit, Institut Pasteur, Inserm U1224, Paris, France
Marie Cherrier
Institut Imagine, Université Paris Descartes, INSERM U1163, Laboratory of Intestinal Immunity, 24 Boulevard du Montparnasse, 75015 Paris, France; Corresponding author
Summary: Innate lymphoid cells (ILCs) have been shown to be significantly affected in the small intestine lamina propria and secondary lymphoid organs (SLOs) of conventional lymphopenic mice. How ILCs are regulated by adaptive immunity in SLOs remains unclear. In T cell-deficient mice, ILC2s are significantly increased in the mesenteric lymph nodes (MLNs) at the expense of CCR6+ ILC3s, which are nonetheless increased in the peripheral lymph nodes (PLNs). Here, we show that T cells regulate lymph node-resident ILCs in a tissue- and subset-specific way. First, reducing microbial colonization from birth restored CCR6+ ILC3s in the MLNs of T cell-deficient mice. In contrast, T cell reconstitution resulted in the contraction of both MLNs ILC2s and PLNs ILC3s, whereas antagonizing microbial colonization from birth had no impact on these populations. Finally, the accumulation of MLNs ILC2s was partly regulated by T cells through stroma-derived IL-33.
