Analysis the protein subunits and fractions of almond meal in comparison to soybean meal and cottonseed meal using SDS-Page electrophoresis and CNCPS method

Abstract

To study the protein subunits and fractions of almond meal in comparison to soybean meal and cottonseed meal, two methods including SDS-Page electrophoresis and CNCPS were used in this research. The results of protein fractions according to CNCPS shows a significant difference (p<0.05) among non-protein nitrogen (A), rapidly degradable true protein (B1), moderately degradable true protein (B2), slowly degradable true protein (B3) and undegradable true protein (C) fractions of almond meal in comparison to soybean meal and cottonseed meal. In regards to SDS-Page electrophoresis results, amandin protein was mainly composed of two major polypeptides with estimated MWs in the range 63-100 kDa (acidic polypeptides) and 20-35 kDa (basic polypeptides). Furthermore, two major polypeptides including β-Conglycinin (three subunits, α, ά, β with MWs of 91.83, 80.49 and 47.16 respectively) and glycinin (two acidic and basic subunits with MWs of 40 and 21.30 kDa respectively) were observed in soybean meal. The pattern of protein subunits in cottonseed meal shows four major polypeptides including Globulin 9S (with MWs of 78.36 and 71.10 kDa), Globulin 5S (with MW of 25.47 kDa) and Albumin 2S (with MW of 15.84 kDa). The results of the current study shows that almond meal have a good nutritive value and can be a good substitute for soybean meal in animal nutrition.

Keywords

• almond meal
• protein subunits
• sds-page electrophoresis
• cncps