Determination of aflatoxin B1 concentration in poultry feed in the poultry farms of Sanandaj using ELISA method

Abstract

Background and Aim: Aflatoxin B1 is a common contaminant of poultry feed and has the highest rate of acute and chronic toxicity among all mycotoxins. Over the past decade, studies have well established the negative effects of aflatoxin B1 on the health of poultry. Therefore, it is necessary to determine the maximum concentration of aflatoxin B1 in poultry feed. Materials and Methods: In this study, in order to determine the aflatoxin B1 concentration in poultry feed, 45 samples were collected via a simple random method during autumn 2018 and winter 2019, and then the concentration of this contaminant was measured by the ELISA method. Results: The experimental results showed that the contamination range in the positive samples was between 6.44 and 34.18 µg/Kg. Moreover, the mean concentration of aflatoxin in contaminated samples was 5.10 µg/Kg. The concentration of contaminant in 46.66% of the samples exceeded the standard limit. Due to higher temperatures and suitable environmental conditions for the growth of fungi, the samples prepared in autumn were more contaminated than those prepared in winter. Conclusion: The level of aflatoxin B1 contamination in poultry feed in Sanandaj is high. In order to prevent the entry of this toxin into the human food cycle regular survey and monitoring of poultry feed by health institutions are necessary.

Keywords

• aflatoxin b1
• poultry feed
• elisa
• sanandaj