Journal of Oral Biology and Craniofacial Research (Oct 2020)
Effect of melatonin versus injectable platelet rich fibrin on critical wound healing in submandibular salivary glands of diabetic rats
Abstract
Background: Diabetes mellitus (DM) has harmful effects on body organs, including submandibular salivary glands (SMGs). It impairs wound healing process that follow sialoadenectomy. Yet there is no complete cure to diabetes, the available medications tend to control the side effects of DM or manage insulin resistance. Herein we tried to investigate the possible effects of injectable platelet rich fibrin (i-PRF) and melatonin on wound healing in diabetic rats. Material and methods: Surgical defects were created in SMGs of 30 rats after confirmation of DM induction. Then rats were randomly and equally allocated into three groups. Group I served as control group; group II received topically applied i-PRF, and group III received topically administrated melatonin. After 28 days all rats were euthanized, and SMGs were processed for histological and biochemical analysis. Results: Both i-PRF and melatonin caused significant reduction of malondialdehyde (P < 0.0001) and caspase-3 (P < 0.001) and significant increase in vascular endothelial growth factors (P = 0.001,0.009 respectively) that increased SMGs regenerative capacity when compared to diabetic group. Melatonin showed superior results regarding the histomorphological structure of SMGs. Conclusion: Melatonin and i-PRF can be possible candidates for improvement of wound healing events in SMGs of diabetic rats.
