PLoS ONE (Jan 2016)

Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells.

  • Tiziana Angrisano,
  • Raffaela Pero,
  • Mariarita Brancaccio,
  • Lorena Coretti,
  • Ermanno Florio,
  • Antonio Pezone,
  • Viola Calabrò,
  • Geppino Falco,
  • Simona Keller,
  • Francesca Lembo,
  • Vittorio Enrico Avvedimento,
  • Lorenzo Chiariotti

DOI
https://doi.org/10.1371/journal.pone.0156671
Journal volume & issue
Vol. 11, no. 6
p. e0156671

Abstract

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Bacterial lipopolysaccharide (LPS) induces release of inflammatory mediators both in immune and epithelial cells. We investigated whether changes of epigenetic marks, including selected histone modification and DNA methylation, may drive or accompany the activation of COX-2 gene in HT-29 human intestinal epithelial cells upon exposure to LPS. Here we describe cyclical histone acetylation (H3), methylation (H3K4, H3K9, H3K27) and DNA methylation changes occurring at COX-2 gene promoter overtime after LPS stimulation. Histone K27 methylation changes are carried out by the H3 demethylase JMJD3 and are essential for COX-2 induction by LPS. The changes of the histone code are associated with cyclical methylation signatures at the promoter and gene body of COX-2 gene.