Data on analysis of OCC-1 transcript levels in pluripotent and differentiated states of P19 cells
Zahra Hosseininia,
Sara Soltanian,
Naser Mahdavi-Shahri,
Hesam Dehghani
Affiliations
Zahra Hosseininia
Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran; Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
Sara Soltanian
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Naser Mahdavi-Shahri
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Hesam Dehghani
Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran; Stem Cells and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran; Corresponding author. Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
We investigated the expression of OCC-1 at mRNA level during retinoic acid (RA) induced differentiation of mouse embryonic carcinoma P19 pluripotent cancer cells by quantitative real time PCR (qPCR). By employing four-fold serial dilutions of P19 cDNA, standard curves were generated for the reference gene (L37) and the gene of interest (OCC-1). PCR efficiencies for L37 and OCC-1 were calculated. Since the amplification efficiencies of these two genes were unequal, the standard curve method was used for the relative quantification of OCC-1. Data analysis revealed that the expression of OCC-1 was reduced by about 69% after 4-day treatment with RA, when significant down-regulation of key pluripotency factors, including OCT4 and Nanog was observed [1]. Keywords: OCC-1, P19, Pluripotency, Differentiation, Retinoic acid
