Purification and Identification of Flavonoids from <i>Prunus mume</i> and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes
Yuxuan Liu,
Mengdie He,
Xinxin Wei,
Qifeng Han,
Xiang Yang,
Gangyi Huang,
Chen Zhang,
Mengqi Wu,
Wenqing Zhang
Affiliations
Yuxuan Liu
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Mengdie He
Zhejiang YingShu Biological Technology Co., Ltd., Hangzhou 311215, China
Xinxin Wei
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Qifeng Han
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Xiang Yang
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Gangyi Huang
Zhejiang YingShu Biological Technology Co., Ltd., Hangzhou 311215, China
Chen Zhang
Zhejiang YingShu Biological Technology Co., Ltd., Hangzhou 311215, China
Mengqi Wu
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Wenqing Zhang
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Prunus mume (Armeniaca mume Sieb. var. mume f. viridicalyx (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in Prunus mume in anti-photoaging cosmetics was developed. Firstly, the flavonoids in Prunus mume were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the Prunus mume purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in Prunus mume as materials for anti-photoaging cosmetics.
