EVALUATION OF FROZEN SEMEN BY A CROSOMAL INTEGRITY AND SPERM CONCENTRATION - TWO VITAL QUALITY PARAMETERS OF MALE FERTILITY IN BOVINES

Abstract

Acrosomal integrity and sperm concentration are two important parameters to assess the quality of frozen semen doses which in terms validates the fertilizing capacity and conception rate. The present study was undertaken to evaluate acrosomal integrity by Giemsa’s stain and sperm concentration of FSS using improved neubauer chamber in Exotic pure Jersey, Crossbred Jersey, Indigenous Gir cattle and Indigenous Murrah buffalo prior to the field use. The overall values of Giemsa’s stain were observed as 73.74±0.31, 18.65±0.33 and 7.79±0.25 percent for Intact Acrosome, Partially Damaged Acrosome and Fully Damaged Acrosome, respectively. Overall values of sperm concentration were 21.98±0.28 million per straw. The study indicated that there was no significant difference (P<0.05) among the breeds and the values mostly correlates with the guideline of Minimum Standard Protocol for Production of bovine semen, 2012 of Govt. of India.

Keywords

• Acrosomal Integrity
• Sperm Concentration
• Giemsa’s staining
• Active volume