Determination of the Carbohydrate Profile and Invertase Activity of Adulterated Honeys after Bee Feeding

Abstract

The higher demand for honey from consumers, combined with its limited availability, has led to different types of honey adulteration, causing substantial economic as well as negative impacts on consumers’ nutrition and health. Therefore, a need has emerged for reliable and cost-effective quality control methods to detect honey adulteration to ensure both the safety and quality of honey. To simulate the process with those applied by beekeepers in real-time, bee colonies were fed with different types of bee feeding (sugar syrup, candy paste and commercial syrup). The produced samples were analyzed for their carbohydrate profile and their invertase activity with the aim to find the effects of bee feeding on the quality of the final product. Honey samples produced after feeding with commercial syrup presented low fructose (22.9 %) and glucose (31.7 %) concentrations and high content of maltose (20.1%), while the samples that came from bee feeding with sugar syrup and candy paste had high concentrations of sucrose (6.2 % and 3.2 %, respectively), exceeding in some cases the legislative limits. Moreover, the samples coming from sugar feeding had lower values of invertase activity, while the group with inverted syrup was clearly discriminated through multi-discriminant analysis. The invertase activity of control samples was found at 153.7 U/kg, which was significantly higher compared to the other groups. The results showed that bee feeding during honey production might lead to adulteration, which can be detected through routine analyses, including the carbohydrate profile and the invertase activity.

Keywords

• bee feeding
• syrup
• honey adulteration
• carbohydrate profile
• HPLC
• invertase activity