Solid Lipid Nanoparticles of Albendazole for Enhancing Cellular Uptake and Cytotoxicity against U-87 MG Glioma Cell Lines
Gregory Marslin,
Karthik Siram,
Xiang Liu,
Vinoth Kumar Megraj Khandelwal,
Xiaolei Shen,
Xiang Wang,
Gregory Franklin
Affiliations
Gregory Marslin
Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Karthik Siram
Department of Pharmaceutics, PSG College of Pharmacy, Coimbatore, Tamilnadu, 641004, India
Xiang Liu
Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Vinoth Kumar Megraj Khandelwal
Department of Translational Pharmacology, Consorzio Mario Negri Sud, Santa Maria Imbaro 66030, Italy
Xiaolei Shen
Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Xiang Wang
Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China
Gregory Franklin
Department of Integrative Plant Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Wielkopolska, Poland
Albendazole (ABZ) is an antihelminthic drug used for the treatment of several parasitic infestations. In addition to this, there are reports on the anticancer activity of ABZ against a wide range of cancer types. However, its effect on glioma has not yet been reported. In the present study, cytotoxicity of ABZ and ABZ loaded solid lipid nanoparticles (ASLNs) was tested in human glioma/astrocytoma cell line (U-87 MG). Using glyceryl trimyristate as lipid carrier and tween 80 as surfactant spherical ASLNs with an average size of 218.4 ± 5.1 nm were prepared by a combination of high shear homogenization and probe sonication methods. A biphasic in vitro release pattern of ABZ from ASLNs was observed, where 82% of ABZ was released in 24 h. In vitro cell line studies have shown that ABZ in the form of ASLNs was more cytotoxic (IC50 = 4.90 µg/mL) to U-87 MG cells compared to ABZ in the free form (IC50 = 13.30 µg/mL) due to the efficient uptake of the former by these cells.
