Ultra-rapid detection of nuclear protein of severe fever with thrombocytopenia syndrome virus by colloidal gold immunochromatography assay
Zhiwei Huang,
Jianhua Li,
Wentao Wei,
Hongyu Li,
Hao Yan,
Ruyi Chen,
Jiaxuan Li,
Xiaotian Tie,
Di Wang,
Guangshang Wu,
Ling Zhang,
Yanjun Zhang,
Keda Chen,
Yongliang Lou
Affiliations
Zhiwei Huang
School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
Jianhua Li
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Wentao Wei
Assure Tech. (Hangzhou) Co., Ltd., Hangzhou, China
Hongyu Li
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Hao Yan
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Ruyi Chen
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Jiaxuan Li
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Xiaotian Tie
Zhejiang Chinese Medical University, Hangzhou, China
Di Wang
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Guangshang Wu
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Ling Zhang
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Yanjun Zhang
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China
Keda Chen
Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, China
Yongliang Lou
School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China
In 2009, severe fever with thrombocytopenia syndrome virus (SFTSV), also known as the Dabie bandavirus (DBV), was first discovered in Henan, China. It is a tick-borne zoonotic virus with a fatality rate ranging from 6% to 30%. Currently, we lack safe and effective vaccines or antiviral drugs to treat SFTSV infection. Therefore, the development of a specific, sensitive, and cost-effective detection method is crucial. Using inactivated SFTSV and recombinant SFTSV nucleocapsid protein (SFTSV-NP), we repeatedly immunized mice with different adjuvants and obtained two monoclonal antibodies against SFTSV-NP, which were used to develop a colloidal gold immunochromatographic assay (ICA) rapid test kit for SFTSV. Compared with nucleic acid testing (gold standard), the ICA test strips are 97.67% accurate in testing clinical serum samples (36 cases of clinical serum samples and seven cases of whole blood samples). The test kit was 100% accurate in detecting different SFTSV strains. No false-positive results were generated when detecting other arboviruses. Therefore, our developed SFTSV test kit conveniently, rapidly, and effectively detects SFTSV.
