Affinity Purification and Molecular Characterization of Angiotensin-Converting Enzyme (ACE)-Inhibitory Peptides from <i>Takifugu flavidus</i>

Yongchang Su; Shicheng Chen; Shuji Liu; Yin Wang; Xiaoting Chen; Min Xu; Shuilin Cai; Nan Pan; Kun Qiao; Bei Chen; Suping Yang; Zhiyu Liu

doi:10.3390/md21100522

Marine Drugs (Sep 2023)

Affinity Purification and Molecular Characterization of Angiotensin-Converting Enzyme (ACE)-Inhibitory Peptides from <i>Takifugu flavidus</i>

Yongchang Su,
Shicheng Chen,
Shuji Liu,
Yin Wang,
Xiaoting Chen,
Min Xu,
Shuilin Cai,
Nan Pan,
Kun Qiao,
Bei Chen,
Suping Yang,
Zhiyu Liu

Affiliations

Yongchang Su: College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Shicheng Chen: Medical Laboratory Sciences Program, College of Health and Human Sciences, Northern Illinois University, DeKalb, IL 60015, USA
Shuji Liu: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Yin Wang: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Xiaoting Chen: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Min Xu: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Shuilin Cai: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Nan Pan: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Kun Qiao: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Bei Chen: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China
Suping Yang: College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Zhiyu Liu: Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China

DOI: https://doi.org/10.3390/md21100522
Journal volume & issue: Vol. 21, no. 10
p. 522

Abstract

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An affinity chromatography filler of CNBr-activated Sepharose 4B-immobilized ACE was used to purify ACE-inhibitory peptides from Takifugu flavidus protein hydrolysate (50 = 93.5 µmol·L−1) was selected. Molecular docking revealed that TLRFALHGME may interact with the active site of ACE through H-bond, hydrophobic, and electrostatic interactions. The total binding energy (ΔGbinding) of TLRFALHGME was estimated to be −82.7382 kJ·mol−1 by MD simulations, indicating the favorable binding of peptides with ACE. Furthermore, the binding affinity of TLRFALHGME to ACE was determined by surface plasmon resonance (SPR) with a Kd of 80.9 µmol, indicating that there was a direct molecular interaction between them. TLRFALHGME has great potential for the treatment of hypertension.

Published in Marine Drugs

ISSN: 1660-3397 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/marinedrugs

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