Resistance Screening to Pepper mild mottle virus Pathotypes in Paprika Cultivars

Abstract

The Paprika plant infected with Pepper mild mottle virus (PMMoV) do not produce commercial fruit as causing necrotic spots symptom on the fruit. Ten cultivars of paprika were analyzed to select the resistance cultivars against PMMoV pathotypes, P1.2 and P1.2.3, using bioassay and genetic markers. L1, L3, and L4 genotypes expressing resistance to the pathotypes existed in those cultivars but L2 genotype did not. L4L4 in cvs. Easy and Magnifico, L4L3 in cvs. Scirocco and Orange glory F1, L4L1 in cv. Special F1, L3L3 in cvs. Fiesta, Piero and Derby, and L3L1 in Cupra and Mazzona F1 were identified with SCAR and CAPS markers. The resistant cvs. to the 2 pathotypes were Magnipico, Easy, Scirocco F1, Orange glory and Special F1 and the susceptible cvs. were Fiesta, Piero, Derby, Cupra and Mazzona F1. The susceptible cvs. of the absence of L4 genotype showed systemic infection when inoculated with PMMoV-P1.2.3. However, those cvs. despite the presence of L3 genotype showed vein necrosis on the inoculated leaf and hypersensitive necrosis symptom on the upper parts when inoculated with PMMoV-P1.2.

Keywords

• Paprika
• Pepper mild mottle virus
• PMMoV