MICROENCAPSULATION BY FREEZE DRYING OF LIMONIN USING β-CYCLODEXTRIN AND ITS STABILITY IN DIFFERENT pH SOLUTION

Abstract

The study aims to determine the effect of limonin core to coating ratio on limonin microencapsulation efficiency and its stability in pH 4 and 7 solution. Encapsulant used was β-cyclodextrin with core to coating ratio 1:10 and 1:20. Microencapsulation process was conducted using freeze dryer. In the analysis, parameters were tested (including encapsulation efficiency) and characterized using a spectrophotometer. Stability of limonin microcapsule in pH 4 and 7 solution was conducted for 12 days. Limonin microencapsulate surface was observed using a scanning electron microscope. Experimental results based on electron microscope showed that Limonin microcapsule has irregular shape and size. Results of the study also revealed that limonin microencapsulation with core to coating ratio of 1:10 had efficiency of 68.14 %, while that with ratio of 1:20 had efficiency of 80.52%.

Keywords

• β-cyclodextrin
• Freeze drying
• Limonin
• Microencapsulation
• pH
• Stability pH