Frontiers in Immunology (Jul 2025)
Advanced immunophenotyping of lymphocyte and monocyte subsets in healthy Australian adults using a novel spectral flow cytometry panel
- Ainsley R. Davies,
- Ainsley R. Davies,
- Kristy Kwong,
- Kristy Kwong,
- Kristy Kwong,
- Zhijia Yu,
- Koula E. M. Diamand,
- Koula E. M. Diamand,
- Fei-Ju Li,
- Fei-Ju Li,
- Laurensia Kannitha,
- Sidra A. Ali,
- Abolfazl Amjadipour,
- Ann-Maree Padarin,
- Michael Devoy,
- Harpreet Vohra,
- Bahar Miraghazadeh,
- Simon H. Jiang,
- Simon H. Jiang,
- Anne Brüstle,
- Nicolas Cherbuin,
- Christopher J. Nolan,
- Christopher J. Nolan,
- Matthew C. Cook,
- Elizabeth E. Gardiner,
- Stuart Read,
- Stuart Read,
- Euan McNaughton,
- Katrina L. Randall,
- Katrina L. Randall,
- Katrina L. Randall
Affiliations
- Ainsley R. Davies
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Ainsley R. Davies
- Phenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia
- Kristy Kwong
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Kristy Kwong
- Phenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia
- Kristy Kwong
- Department of Medicine, University of Cambridge, Cambridge, United Kingdom
- Zhijia Yu
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Koula E. M. Diamand
- Phenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia
- Koula E. M. Diamand
- ANU Centre for Therapeutic Discovery, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia
- Fei-Ju Li
- Phenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia
- Fei-Ju Li
- Cytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Laurensia Kannitha
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Sidra A. Ali
- Division of Genome Science and Cancer, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Abolfazl Amjadipour
- Division of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Ann-Maree Padarin
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Michael Devoy
- Cytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Harpreet Vohra
- Cytometry, Histology and Advanced Spatial Multiomics, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Bahar Miraghazadeh
- Division of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Simon H. Jiang
- Division of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Simon H. Jiang
- Department of Renal Medicine, The Canberra Hospital, Canberra, ACT, Australia
- Anne Brüstle
- Division of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Nicolas Cherbuin
- National Centre for Epidemiology and Population Health, Australian National University, Canberra, ACT, Australia
- Christopher J. Nolan
- Division of Immunology and Infectious Disease, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Christopher J. Nolan
- 0School of Medicine and Psychology, The Australian National University, Canberra, ACT, Australia
- Matthew C. Cook
- Department of Medicine, University of Cambridge, Cambridge, United Kingdom
- Elizabeth E. Gardiner
- Division of Genome Science and Cancer, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Stuart Read
- Phenomics Translation Initiative, John Curtin School of Medical Research, Australian Phenomics Facility, The Australian National University, Canberra, ACT, Australia
- Stuart Read
- 1South Australian Health and Medical Research Institute, Adelaide, SA, Australia
- Euan McNaughton
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Katrina L. Randall
- Canberra Clinical Phenomics Service, John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia
- Katrina L. Randall
- 0School of Medicine and Psychology, The Australian National University, Canberra, ACT, Australia
- Katrina L. Randall
- 2Department of Immunology, and Department of Immunopathology, ACT Pathology, Canberra Health Services, Canberra, ACT, Australia
- DOI
- https://doi.org/10.3389/fimmu.2025.1577206
- Journal volume & issue
-
Vol. 16
Abstract
IntroductionLymphocytes play pivotal roles in disease pathogenesis and can be used as potential biomarkers for various immunological conditions. Yet, current flow cytometry methods used in clinical settings are often only capable of measuring between four to eight distinct lymphocyte populations. The purpose of our study was to measure many lymphocyte and monocyte populations from a single sample, with the long-term aim of validating our assay for diagnostic use in the Australian regulatory environment.MethodsWe designed and optimised a novel 30-colour lymphocyte immunophenotyping panel tailored for use on a 3-laser (V-B-R) spectral flow cytometer. This panel measures over 50 lymphocyte and monocyte populations.ResultsIn this report we present data derived from 148 healthy individuals.DiscussionThis lays the groundwork for future clinical application of spectral flow cytometry tests and offers a more comprehensive approach to lymphocyte and monocyte analysis with future implications for disease diagnosis and monitoring.
Keywords