Comparative assessment of total phenolic content and in vitro antioxidant activities of bark and leaf methanolic extracts of Manilkara hexandra (Roxb.) Dubard

Abstract

Manilkara hexandra is a traditionally used medicinal plant. The aim of the present study was to analyze in vitro antioxidant potentials of the methanolic leaf (LMEMH) and bark (BMEMH) extracts of M. hexandra in relation to total phenolics. Here, in vitro antioxidant activities of the extracts were tested through 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, ferric ion reducing antioxidant power (FRAP), hydroxyl radical scavenging (HRS), and the total antioxidant activity (TAC) assays. The EC50 of DPPH free radical scavenging with ascorbic acid, LMEMH, and BMEMH were calculated as 19.06 ± 2.2, 46.62 ± 6.13, and 88.66 ± 24.07 µg/mL respectively. The EC50 of the Fe3+ ion reduction by ascorbic acid, LMEMH, and BMEMH were calculated respectively as 54.06 ± 5.00, 147.45 ± 22.46 and 480.34 ± 91.65 µg/mL. The LMEMH shows more HRS activity and the total phenolic content than the BMEMH. In conclusion, the leaf methanolic extract of M. hexandra has the comparatively more in vitro antioxidant potentials and total phenolics content than its bark methanolic extract. Keywords: Antioxidant, DPPH, FRAP, Reactive oxygen species, Manilkara hexandra