Malaria Journal (Nov 2011)

Use of a pLDH-based dipstick in the diagnostic and therapeutic follow-up of malaria patients in Mali

  • Ouattara Amed,
  • Doumbo Safiatou,
  • Saye Renion,
  • Beavogui Abdoul H,
  • Traoré Boubacar,
  • Djimdé Abdoulaye,
  • Niangaly Amadou,
  • Kayentao Kassoum,
  • Diallo Mouctar,
  • Doumbo Ogobara K,
  • Thera Mahamadou A

DOI
https://doi.org/10.1186/1475-2875-10-345
Journal volume & issue
Vol. 10, no. 1
p. 345

Abstract

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Abstract Background Malaria is a major public health problem in Mali and diagnosis is typically based on microscopy. Microscopy requires a well trained technician, a reliable power source, a functioning microscope and adequate supplies. The scarcity of resources of community health centres (CHC) does not allow for such a significant investment in only one aspect of malaria control. In this context, Rapid Diagnostic Tests (RDTs) may improve case management particularly in remote areas. Methods This multicentre study included 725 patients simultaneously screened with OptiMal-IT test and thick smears for malaria parasite detection. While evaluating the therapeutic efficacy of choroquine in 2 study sites, we compared the diagnostic values of thick smear microscopy to OptiMal-IT test applying the WHO 14 days follow-up scheme using samples collected from 344 patients. Results The sensitivity and the specificity of OptiMal-IT compared to thick smear was 97.2% and 95.4%, whereas the positive and negative predictive values were 96.7 and 96.1%, respectively. The percent agreement between the two diagnostic tests was 0.93. The two tests were comparable in detecting malaria at day 0, day 3 and day 14. The only difference was observed at day 7 due to high gametocytemia. Subjectively, health care providers found OptiMal-IT easier to use and store under field conditions. Conclusion OptiMal-IT test revealed similar results when compared to microscopy which is considered the gold standard for malaria diagnostics. The test was found to have a short processing time and was easier to use. These advantages may improve malaria case management by providing a diagnostic and drug efficacy follow-up tool to peripheral health centres with limited resources.

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