Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’)

Abstract

A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’) was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS) containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on ¼MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.

Keywords

• nodal culture
• organogenesis
• Rosa hybrida
• rose