Emerging Infectious Diseases (Jun 2007)

Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

  • Shanthi Jayawardena,
  • Chung Y. Cheung,
  • Ian Barr,
  • Kwok H. Chan,
  • Honglin Chen,
  • Yi Guan,
  • J.S. Malik Peiris,
  • Leo L.M. Poon

DOI
https://doi.org/10.3201/eid1306.061572
Journal volume & issue
Vol. 13, no. 6
pp. 899 – 901

Abstract

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We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.

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