Nature Communications (Dec 2017)
Crystal structure of an RNA-cleaving DNAzyme
- Hehua Liu,
- Xiang Yu,
- Yiqing Chen,
- Jing Zhang,
- Baixing Wu,
- Lina Zheng,
- Phensinee Haruehanroengra,
- Rui Wang,
- Suhua Li,
- Jinzhong Lin,
- Jixi Li,
- Jia Sheng,
- Zhen Huang,
- Jinbiao Ma,
- Jianhua Gan
Affiliations
- Hehua Liu
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- Xiang Yu
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- Yiqing Chen
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- Jing Zhang
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- Baixing Wu
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
- Lina Zheng
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
- Phensinee Haruehanroengra
- Department of Chemistry and The RNA Institute, University at Albany, State University of New York
- Rui Wang
- Department of Chemistry and The RNA Institute, University at Albany, State University of New York
- Suhua Li
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- Jinzhong Lin
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
- Jixi Li
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- Jia Sheng
- Department of Chemistry and The RNA Institute, University at Albany, State University of New York
- Zhen Huang
- Department of Chemistry, Georgia State University
- Jinbiao Ma
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University
- Jianhua Gan
- State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan University
- DOI
- https://doi.org/10.1038/s41467-017-02203-x
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 10
Abstract
RNA-cleaving DNA enzymes are catalytic DNA that can cleave RNA in a sequence-specific manner. Here, the authors report three crystal structures of the 8–17 DNAzyme that include the pre-catalytic state of the RNA cleavage reaction, providing insight into the catalytic mechanism and may guide the rational design of DNAzymes.
