The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant

Abstract

The use of the stable free radical diphenylpicrylhydrazyl (DPPH) to estimate the activity of antioxidants is reviewed. Current applications of the method are examined, particularly the use of the parameter EC50 (substrate concentration to produce 50% reduction of the DPPH). Some recommendations are made as to the most suitable ways of carrying out this assay and evaluating the data produced.

Keywords

• DPPH
• diphenylpicrylhydrazyl
• free radical
• antioxidant activity