Comparative analysis of minor histocompatibility antigens genotyping methods

Abstract

The wide range of techniques could be employed to find mismatches in minor histocompatibility antigens between transplant recipients and their donors. In the current study we compared three genotyping methods based on polymerase chain reaction (PCR) for four minor antigens. Three of the tested methods: allele-specific PCR, restriction fragment length polymorphism and real-time PCR with TaqMan probes demonstrated 100% reliability when compared to Sanger sequencing for all of the studied polymorphisms. High resolution melting analysis was unsuitable for genotyping of one of the tested minor antigens (HA-1) as it has linked synonymous polymorphism. Obtained data could be used to select the strategy for large-scale clinical genotyping.

Keywords

• minor histocompatibility antigen
• single nucleotide polymorphism
• allogeneic hematopoietic stem cells transplantation
• genotyping
• allelic discrimination