Reaction of Energetic Metabolism and Antioxidant Enzymes to Hypoxia and Reoxygenation in Deep Water Amphipods Ommatogammarus carneolus melanophthalmus from Lake Baikal

Abstract

The aim of the current study was to estimate the effects of acute hypoxia and reoxygenation on energy metabolism and activities of antioxidant enzymes as markers of oxidative stress in Baikal deep water amphipods Ommatogammarus carneolus melanophthalmus. The amphipods were exposed under hypoxic conditions at 4 mg of О2/L during 3 hours with following normoxic recovery at 9-11 mg of О2/L for 3 hours. There were no significant changes in most of studied parameters except glucose content. High lactate content and antioxidant enzymes activity in control group and absence of their reaction to hypoxia and reoxygenation can be explained by difficulties with adaptation to atmospheric pressure in O. carneolus melanophthalmus or by low level of hypoxia for studied species.

Keywords

• baikal
• catalase
• deep-water amphipods
• glucose
• glutathione s-transferase
• glycogen
• hypoxia
• lactate
• ommatogammarus carneolus melanophthalmus
• peroxidase