WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy

Daniela Bakula; Amelie J. Müller; Theresia Zuleger; Zsuzsanna Takacs; Mirita Franz-Wachtel; Ann-Katrin Thost; Daniel Brigger; Mario P. Tschan; Tancred Frickey; Horst Robenek; Boris Macek; Tassula Proikas-Cezanne

doi:10.1038/ncomms15637

Nature Communications (May 2017)

WIPI3 and WIPI4 β-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy

Daniela Bakula,
Amelie J. Müller,
Theresia Zuleger,
Zsuzsanna Takacs,
Mirita Franz-Wachtel,
Ann-Katrin Thost,
Daniel Brigger,
Mario P. Tschan,
Tancred Frickey,
Horst Robenek,
Boris Macek,
Tassula Proikas-Cezanne

Affiliations

Daniela Bakula: Department of Molecular Biology, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen
Amelie J. Müller: Department of Molecular Biology, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen
Theresia Zuleger: Department of Molecular Biology, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen
Zsuzsanna Takacs: Department of Molecular Biology, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen
Mirita Franz-Wachtel: Proteome Center Tuebingen, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen
Ann-Katrin Thost: Department of Molecular Biology, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen
Daniel Brigger: Division of Experimental Pathology, Institute of Pathology, University of Bern
Mario P. Tschan: Division of Experimental Pathology, Institute of Pathology, University of Bern
Tancred Frickey: Department of Biology, Applied Bioinformatics, Konstanz University
Horst Robenek: Institute of Experimental Musculoskeletal Medicine, University Hospital Muenster
Boris Macek: International Max Planck Research School 'From Molecules to Organisms', Max Planck Institute for Developmental Biology and Eberhard Karls University Tuebingen
Tassula Proikas-Cezanne: Department of Molecular Biology, Interfaculty Institute of Cell Biology, Eberhard Karls University Tuebingen

DOI: https://doi.org/10.1038/ncomms15637
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 18

Abstract

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During autophagy, AMPK and mTOR associate with ULK1 and regulate phosphatidylinositol 3-phosphate (PtdIns3P) production that mediates autophagosome formation via WIPI proteins. Here the authors show WIPI3 and WIPI4 have a scaffolding function upstream of PtdIns3P production and have a role in the PtdIns3P effector function of WIPI1-WIPI2 at nascent autophagosomes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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