AIP Advances (Jan 2016)

Influence of α-amylase template concentration on systematic entrapment of highly stable and monodispersed colloidal gold nanoparticles

  • A. Nitthin Ananth,
  • A. Nimrodh Ananth,
  • Sujin P. Jose,
  • S. Umapathy,
  • T. Mathavan

DOI
https://doi.org/10.1063/1.4939849
Journal volume & issue
Vol. 6, no. 1
pp. 015005 – 015005-8

Abstract

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Nano gold / α-amylase colloidal dispersions of profound stability were made using simple procedure with a conventional reducing agent. The surface plasmon resonance of the gold nanocrystals was used to quantify the extent of the dispersion stability and functionalization. It is found that the reduced gold nanoparticles were trapped into the protein network without denaturation the structure of α-amylase protein. This kind of entrapment of particles into the protein network prevents clustering of individual gold nanoparticles (6.42 nm ± 0.92 nm) by acting as a natural spacer. Systematic entrapment was facilitated by the affinity of gold to the sulfur moieties (Au-S) in the protein structure.