Ultrasound-Assisted Extraction of Phenolic Compounds from Different Maturity Stages and Fruit Parts of <i>Cordia dodecandra</i> A. DC.: Quantification and Identification by UPLC-DAD-ESI-MS/MS

Abstract

In the present work, the total phenolic content (TPC), total flavonoids content (TFC), antioxidant activity, and phenolic profile from pulp (PU) and peel (PE) extracts obtained from the ciricote (Cordia dodecandra A. DC.) fruit by ultrasound-assisted extraction (UAE) in immature (IM), semimature (SM), and mature (MM) stages were investigated. The effect of the diameter of the ultrasonic probe in the IM stage was also evaluated. The TPC and antioxidant activity in IM fruit extracts by UAE increased up to 11.01 and 23.82 times, respectively, compared to the maceration method. The main phenolic compounds in the PE of IM fruit identified by UPLC-DAD-ESI-MS/MS were quantified as caffeic acid, rutin, and rosmarinic acid, distributed as 45.82, 41.45, and 12.72%, respectively. The PE extracts of IM fruit obtained with the 3 mm diameter probe had 1.27, 2.44, and 1.37 times the TPC (19.93 ± 0.28 mg GAE (Gallic equivalents) g−1 dw), TFC (34.85 ± 4.99 mg RE (Rutin equivalents) g−1 dw), and antioxidant activity (122.09 ± 17.09 µTE (Trolox equivalents) g−1 (DPPH)), respectively, compared to those obtained with a 13 mm diameter probe. The results obtained suggest the use of the ciricote native fruit as a source of bioactive compounds, directly as fresh fruit or processed, thus helping to increase its production and consumption.

Keywords

• phenolic compounds
• ciricote fruit
• ultrasonic probe diameter
• antioxidant activity
• rosmarinic acid
• rutin