CANARY Technology: Working Principles of Biosensor, Detection of Infectious Pathogens

Abstract

Reusable biosensors for the detection of biochemical analytes are widely used in clinical and laboratory practice. However, biosensors for the detection of pathogenic microorganisms are still under development or implementation. One of these devices is CANARY biosensor (Cellular Analysis and Notification of Antigen Risks and Yields), used by the US Army to indicate pathogenic biological agents. The aim of this article is to consider operating principles and molecular-biological foundations of CANARY biosensor, to analyze the possible directions of work and the prospects for creating domestically made biosensors based on eukaryotic cells. The concept of CANARY is that its receptor component is a B-lymphocyte, modified using genetic engineering, which carries specific IgM-like B-cell receptors on the surface of the cytoplasmic membrane. These cells are able to specifically recognize the target antigen and generate a photosignal through the aequorin protein. Currently, biosensors are already created for the detection of causative agents of plague (100–1000 CFU / ml), tularemia (100 CFU / ml), anthrax (100–500 spores / ml), smallpox (<500 CFU / ml), some toxins (ricin – 3 ng / ml, botulinum toxin – 16 pg / ml). They are based on the CANARY biosensor. Due to high sensitivity and specificity of this method, the relative simplicity and high speed of analysis of one sample, the possibility of analyzing aerosol samples, this technology should be considered as a promising basis for the creation of domestically made biological sensors to detect hazardous biological agents in biological samples, water, food, ecological samples and in aerosols. The deterioration of the global epidemic situation caused by the spread of various strains of SARS-CoV-2 makes sensors based on CANARY technology especially relevant. To create a domestic analogue of such a biosensor, close cooperation with scientific institutions that specialize in molecular genetics and manufacturers of laboratory equipment is required.

Keywords

• aerosol
• b-lymphoblast
• b lymphocytes
• reagent-free analysis
• biosensor
• igm-like b-cell receptors
• indication of pathogenic biological agents
• canary technology
• aequorin