Frontiers in Microbiology (Aug 2021)
An Auto-Regulating Type II Toxin-Antitoxin System Modulates Drug Resistance and Virulence in Streptococcus suis
- Qibing Gu,
- Qibing Gu,
- Qibing Gu,
- Peijuan He,
- Peijuan He,
- Peijuan He,
- Dan Wang,
- Dan Wang,
- Dan Wang,
- Jiale Ma,
- Jiale Ma,
- Jiale Ma,
- Xiaojun Zhong,
- Xiaojun Zhong,
- Xiaojun Zhong,
- Xiaojun Zhong,
- Yinchu Zhu,
- Yinchu Zhu,
- Yinchu Zhu,
- Yinchu Zhu,
- Yue Zhang,
- Yue Zhang,
- Yue Zhang,
- Yue Zhang,
- Qiankun Bai,
- Qiankun Bai,
- Qiankun Bai,
- Zihao Pan,
- Zihao Pan,
- Zihao Pan,
- Huochun Yao,
- Huochun Yao,
- Huochun Yao
Affiliations
- Qibing Gu
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Qibing Gu
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Qibing Gu
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Peijuan He
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Peijuan He
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Peijuan He
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Dan Wang
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Dan Wang
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Dan Wang
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Jiale Ma
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Jiale Ma
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Jiale Ma
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Xiaojun Zhong
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Xiaojun Zhong
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Xiaojun Zhong
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Xiaojun Zhong
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Yinchu Zhu
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Yinchu Zhu
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Yinchu Zhu
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Yinchu Zhu
- Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
- Yue Zhang
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Yue Zhang
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Yue Zhang
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Yue Zhang
- College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China
- Qiankun Bai
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Qiankun Bai
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Qiankun Bai
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Zihao Pan
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Zihao Pan
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Zihao Pan
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- Huochun Yao
- College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
- Huochun Yao
- Key Laboratory of Animal Bacteriology, Ministry of Agriculture, Nanjing, China
- Huochun Yao
- OIE Reference Laboratory for Swine Streptococcosis, Nanjing, China
- DOI
- https://doi.org/10.3389/fmicb.2021.671706
- Journal volume & issue
-
Vol. 12
Abstract
Toxin-antitoxin (TA) systems are ubiquitous genetic elements that play an essential role in multidrug tolerance and virulence of bacteria. So far, little is known about the TA systems in Streptococcus suis. In this study, the Xress-MNTss TA system, composed of the MNTss toxin in the periplasmic space and its interacting Xress antitoxin, was identified in S. suis. β-galactosidase activity and electrophoretic mobility shift assay (EMSA) revealed that Xress and the Xress-MNTss complex could bind directly to the Xress-MNTss promoter as well as downregulate streptomycin adenylyltransferase ZY05719_RS04610. Interestingly, the Xress deletion mutant was less pathogenic in vivo following a challenge in mice. Transmission electron microscopy and adhesion assays pointed to a significantly thinner capsule but greater biofilm-formation capacity in ΔXress than in the wild-type strain. These results indicate that Xress-MNTss, a new type II TA system, plays an important role in antibiotic resistance and pathogenicity in S. suis.
Keywords
