Nature Communications (Aug 2020)

A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis

  • Matthew J. Amicucci,
  • Eshani Nandita,
  • Ace G. Galermo,
  • Juan Jose Castillo,
  • Siyu Chen,
  • Dayoung Park,
  • Jennifer T. Smilowitz,
  • J. Bruce German,
  • David A. Mills,
  • Carlito B. Lebrilla

DOI
https://doi.org/10.1038/s41467-020-17778-1
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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While mass spectrometry-based proteomics largely relies on digesting proteins into peptides, there is no equivalent strategy for polysaccharide analysis. Here, the authors develop a chemical approach to break down poly- into oligosaccharides and present a workflow to identify polysaccharides by oligosaccharide fingerprinting.