Plasma Macro and Trace Element Levels of Female Rats Vaccinated with GnRH Hormone on Reproductive

Abstract

INTRODUCTION: There is a significant relationship between hormones and trace elements. Trace elements can cause harmful and beneficial effects on animal and human life depending on their concentration. In this study, it was aimed to investigate the changes in macro- and trace element levels in blood plasmas of 6th and 16th months of GnRH vaccination (Repro-Bloc ™) female rats. METHODS: 50 female Wistar Albino rats were used in the study. Each rat in the experimental group was administered a single 200 μl intramuscular dose of GnRH. The levels of Cu, Fe, Mn, Zn, Se, K, P, Ca, and Mg elements were determined by analyzing the collected plasma samples using inductively coupled plasma-optical emission spectroscopy (ICP-OES). RESULTS: It was determined that zinc, manganese and selenium levels of the trace elements showed statistically significant changes in the first 6 months and Cu and Mn levels were statistically significant (P <0.05). In the macro elements, Ca, P elements in the first 6 months period and Ca and Mg element levels in 16 months period gave statistically significant results (P <0.05). DISCUSSION AND CONCLUSION: In the study, it was revealed that the GnRH vaccine administered to rats affected macro-element and trace element levels.

Keywords

• trace element
• macro element
• gnrh vaccine
• rat
• icp-oes