International Journal of General Medicine (Dec 2021)
Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction
Abstract
Shuo Wang, Yuying Liu, Xitian Hu, Xiaolei Zhang, Lei Xu, Yan Yang, Rubing Wu, Enmao Wang, Tianjie Lv Department of Cardiovasology, Shijiazhuang People’s Hospital, Shijiazhuang, People’s Republic of ChinaCorrespondence: Xitian HuDepartment of Cardiovasology, Shijiazhuang People’s Hospital, No. 9 Fangbei Road, Shijiazhuang, Hebei, 050000, People’s Republic of ChinaTel +86-17603119015Email [email protected]: Myocardial fibrosis (MF) after acute myocardial infarction (AMI) ultimately results in heart failure, which is a serious threat to human life. This study aimed to excavate critical biomarkers associated with MF after AMI.Materials and Methods: RNA-sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in AMI and MF after AMI.Results: Abundant DEmRNAs, DEmiRNAs and DElncRNAs were identified in AMI and MF after AMI. The ceRNA network, which contained 9 lncRNA-miRNA pairs and 9 miRNA-mRNA pairs, was acquired. In AMI, all candidate markers generally exhibited the same pattern as that in our RNA-seq results; while in MF after AMI, except for CENPB, JAK2 and hsa-miR-197-3p, the expression of the others in the qRT-PCR results exhibited the same pattern as that in our RNA-seq results.Conclusion: We speculated that LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs may serve as potential biomarkers in MF after AMI.Keywords: acute myocardial infarction, myocardial fibrosis, lncRNA, ceRNA network
