Quantitative proteome and lysine succinylome characterization of zinc chloride smoke-induced lung injury in mice
Rui Zhou,
Zhiwei Tu,
Daishi Chen,
Wanmei Wang,
Shuzi Liu,
Linjun She,
Zhan Li,
Jihong Liu,
Yabin Li,
Yu Cui,
Pan Pan,
Fei Xie
Affiliations
Rui Zhou
The First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, China
Zhiwei Tu
National Center for Protein Sciences (Beijing), Institute of Lifeomics, 102206, Beijing, China
Daishi Chen
Department of Otorhinolaryngology, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, 515100, Shenzhen, Guangdong, China
Wanmei Wang
Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, 100850, Beijing, China
Shuzi Liu
College of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, China
Linjun She
The First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, China
Zhan Li
The First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, China
Jihong Liu
The First Affiliated Hospital of Henan University of Chinese Medicine, 450000, Zhengzhou, Henan, China
Yabin Li
College of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, China
Yu Cui
National Center for Protein Sciences (Beijing), Institute of Lifeomics, 102206, Beijing, China; State Key Laboratory of Proteomics, 102206, Beijing, China; Corresponding author. National Center for Protein Sciences (Beijing), Institute of Lifeomics, 102206, Beijing, China.
Pan Pan
College of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, China; Corresponding author. College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, 100048, Beijing, China.
Fei Xie
College of Pulmonary and Critical Care Medicine, The First Medical Center of Chinese PLA General Hospital, 100048, Beijing, China; Corresponding author. College of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, 100048, Beijing, China.
The inhalation of zinc chloride (ZnCl2) smoke is one of common resources of lung injury, potentially resulting in severe pulmonary complications and even mortality. The influence of ZnCl2 smoke on lysine succinylation (Ksucc) in the lungs remains uncertain. In this study, we used a ZnCl2 smoke inhalation mouse model to perform global proteomic and lysine succinylome analyses. A total of 6781 Ksucc sites were identified in the lungs, with injured lungs demonstrating a reduction to approximately 2000 Ksucc sites, and 91 proteins exhibiting at least five differences in the number of Ksucc sites. Quantitative analysis revealed variations in expression of 384 proteins and 749 Ksucc sites. The analysis of protein-protein interactions was conducted for proteins displaying differential expression and differentially expressed lysine succinylation. Notably, proteins with altered Ksucc exhibited increased connectivity compared with that in differentially expressed proteins. Beyond metabolic pathways, these highly connected proteins were also involved in lung injury-associated pathological reactions, including processes such as focal adhesion, adherens junction, and complement and coagulation cascades. Collectively, our findings contribute to the understanding of the molecular mechanisms underlaying ZnCl2 smoke-induced lung injury with a specific emphasis on lysine succinylation. These findings could pave the way for targeted interventions and therapeutic strategies to mitigate severe pulmonary complications and mortality associated with such injuries in humans.
