Characterization and comparative analyses of transcriptomes of cloned and in vivo fertilized porcine pre-implantation embryos
Xiaoyan He,
Cheng Tan,
Zicong Li,
Chengfa Zhao,
Junsong Shi,
Rong Zhou,
Xingwang Wang,
Gelong Jiang,
Gengyuan Cai,
Dewu Liu,
Zhenfang Wu
Affiliations
Xiaoyan He
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Cheng Tan
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Zicong Li
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Chengfa Zhao
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Junsong Shi
Wen's Group Academy, Wen's Foodstuff Group Co., Ltd, Yunfu 527400, China, China
Rong Zhou
Wen's Group Academy, Wen's Foodstuff Group Co., Ltd, Yunfu 527400, China, China
Xingwang Wang
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Gelong Jiang
Wen's Group Academy, Wen's Foodstuff Group Co., Ltd, Yunfu 527400, China, China
Gengyuan Cai
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Dewu Liu
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Zhenfang Wu
National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Somatic cell nuclear transfer (SCNT) is the only method known to rapidly reprogram differentiated cells into totipotent embryos. Most cloned embryos become arrested before implantation and the details of the underlying molecular mechanism remain largely unknown. Dynamic regulation of the transcriptome is a key molecular mechanism driving early embryonic development. Here, we report comprehensive transcriptomic analysis of cloned embryos (from Laiwu and Duroc pigs) and in vivo fertilized embryos (from Duroc pigs) using RNA-sequencing. Comparisons between gene expression patterns were performed according to differentially expressed genes, specific-expressed genes, first-expressed genes, pluripotency genes and pathway enrichment analysis. In addition, we closely analyzed the improperly expressed histone lysine methyltransferases and histone lysine demethylases during cell reprogramming in cloned embryos. In summary, we identified altered gene expression profiles in porcine cloned pre-implantation embryos in comparison to normal in vivo embryos. Our findings provide a substantial framework for further discovery of the epigenetic reprogramming mechanisms in porcine SCNT embryos.
