CaRuby-Nano: a novel high affinity calcium probe for dual color imaging
Mayeul Collot,
Christian D Wilms,
Asma Bentkhayet,
Païkan Marcaggi,
Kiri Couchman,
Serge Charpak,
Stéphane Dieudonné,
Michael Häusser,
Anne Feltz,
Jean-Maurice Mallet
Affiliations
Mayeul Collot
Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France
Christian D Wilms
Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom
Asma Bentkhayet
Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France
Païkan Marcaggi
Institut de Biologie de l'École Normale Supérieure, Paris, France
Kiri Couchman
Institut National de la Santé et de la Recherche Médicale, Paris, France; Laboratory of Neurophysiology and New Microscopies, Université Paris Descartes, Paris, France
Serge Charpak
Institut National de la Santé et de la Recherche Médicale, Paris, France; Laboratory of Neurophysiology and New Microscopies, Université Paris Descartes, Paris, France
Stéphane Dieudonné
Institut de Biologie de l'École Normale Supérieure, Paris, France
Michael Häusser
Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom
Anne Feltz
Institut de Biologie de l'École Normale Supérieure, Paris, France
Jean-Maurice Mallet
Laboratory of Biomolecules, UPMC Université Paris 06, Ecole Normale Supérieure, Paris, France
The great demand for long-wavelength and high signal-to-noise Ca2+ indicators has led us to develop CaRuby-Nano, a new functionalizable red calcium indicator with nanomolar affinity for use in cell biology and neuroscience research. In addition, we generated CaRuby-Nano dextran conjugates and an AM-ester variant for bulk loading of tissue. We tested the new indicator using in vitro and in vivo experiments demonstrating the high sensitivity of CaRuby-Nano as well as its power in dual color imaging experiments.
