Nature Communications (Aug 2018)

Automated single-molecule imaging in living cells

  • Masato Yasui,
  • Michio Hiroshima,
  • Jun Kozuka,
  • Yasushi Sako,
  • Masahiro Ueda

DOI
https://doi.org/10.1038/s41467-018-05524-7
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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Large scale live cell screens often lack single-molecule resolution. Here the authors present an artificial intelligence-assisted TIRF microscope with automated cell searching and focusing, and use it for high-throughput single-molecule imaging of EGFR dynamics in response to various stimuli.