Acta Biomedica Scientifica (May 2018)

Features of lipid peroxidation and antioxidant protection in Mongoloid men, carriers of non-functional polymorphisms of GSTT1 and GSTM1 genes, with different status of fertility

  • N. A. Kurashova,
  • T. A. Bairova,
  • M. I. Dolgikh,
  • E. V. Osipova,
  • B. G. Dashiev,
  • A. V. Labygina,
  • I. N. Gutnik,
  • I. M. Mikhalevich,
  • L. I. Kolesnikova

DOI
https://doi.org/10.29413/ABS.2018-3.3.19
Journal volume & issue
Vol. 3, no. 3
pp. 121 – 126

Abstract

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Background. Genetic factors cause 30-50 % of cases of male infertility different forms. We have conducted the investigation of the lipid peroxidation and antioxidant protection system components, associated with the biotransformation genes polymorphisms, in men with infertility. Aims: to study parameters of lipid peroxidation and antioxidant defense system associated with polymorphisms of biotransformation genes in infertile Mongoloid men. Materials and methods. The main group included 143 men of reproductive age from Mongoloid population who came to the Public Health Institution "Republican Perinatal Center" in Ulan-Ude with the problem of one year and more infertility after the marriage. Fifty-three almost healthy males with proven fertility were included in the control group. Blood samples of all men were genotyped by glutathione S-transferase (GSTP1, GSTM1, GSTT1) gene family and activity of glutathione system enzymes was determined. Results. In the fertile Mongoloids with non-functional genotype GSTT1(0/0)/GSTM1(0/0) we have established an increase concentration of a-tocopherol in serum, activity of glutathione peroxidase and a decrease in glutathione-S-transferase, as well as a decrease in the concentration of a-tocopherol. In infertile Mongoloids with non-functional GSTT1(0/0)/ GSTM1 (0/0) genotypes, an increase in the tiobarbituric acid reactants, glutathione peroxidase and a decrease in the concentration of a-tocopherol was found in the ejaculate; we also detected a decrease in the concentration of a-tocopherol, the level of oxidized glutathione and glutathione-s-transferase activity. Conclusions. Genetically determined peculiarities of functioning of the xenobiotic biotransformation system make each individual unique with regard to their adaptive capacity - resistance or sensibility to the damaging exo- and endogenous factors.

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