Application of factorial experimental design on the optical absorption from glucose–insulin samples in mid-infrared spectroscopy

Abstract

An important challenge to be addressed in the search for an optically based, non-invasive blood glucose quantification method, is related to the spectral interference between glucose and insulin in the mid-infrared region. This work proposes the use of a factorial experimental design in order to understand the optical overlapping of these compounds. The results show how in the 1100–950 cm−1 range, glucose has a greater contribution than insulin to the absorption spectrum, without a significant effect from their interaction. These results and the use of the proposed technique could bring in interesting insights for biomedical applications. Keywords: Non-invasive blood glucose quantification, Glucose - insulin, Factorial experimental design, mid-IR spectroscopy