Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

Diming Zhang; Emily Redington; Yiyang Gong

doi:10.1038/s42003-021-02452-z

Communications Biology (Jul 2021)

Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins

Diming Zhang,
Emily Redington,
Yiyang Gong

Affiliations

Diming Zhang: Department of Biomedical Engineering, Duke University
Emily Redington: Department of Biomedical Engineering, Duke University
Yiyang Gong: Department of Biomedical Engineering, Duke University

DOI: https://doi.org/10.1038/s42003-021-02452-z
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 12

Abstract

Read online

Zhang, Redington & Gong develop FRET-based ratiometric calcium sensors with pairs of green and red fluorescent proteins. Their best performers, Twitch-GR and Twitch-NR inherit the superior photophysical properties of their constituent fluorescent proteins, enabling them to outperform existing ratiometric calcium sensors in brightness, photobleaching, and signal fidelity metrics.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal